Laboratory Safety Manual
Reviewed May 2012
Chapter 1: Emergency Response
Section 1.3 - Biohazard Spills
C. Cytotoxic/Antineoplastic Spills
- General Procedures
- Personnel Contamination
Overt contamination of gloves or gowns, or direct skin or eye contact should be treated as
- Clean-up of Small Spills
Spills of less than 5 ml or 5 gm outside a hood should be cleaned immediately by personnel
wearing gowns, double surgical latex gloves, and eye protection.
- Clean-up of Large Spills
For spills of amounts larger than 5 ml or 5 gm, the spread should be limited by gently
covering with absorbent sheets of spill-control pads or pillows or, if a powder is
involved, with damp cloths or towels. Be sure not to generate aerosols. Access to the
spill areas should be restricted.
Spills in Hoods
If the spill occurred in a glove box, clean bench or biological safety
cabinet, the HEPA filter (contained in the cabinet) is more than likely
contaminated. Label the unit
"Do Not Use--Contaminated With (name of substance)." The HEPA filter and filter
cabinet must be decontaminated and the filter changed and properly disposed of. This
procedure may require the services of an outside contractor trained in the use of
specialized personal protective equipment.
Spill kits, clearly labeled, should be kept in or near preparation and
administrative areas. It is suggested that kits include a respirator**,
chemical splash goggles, two pairs of gloves, two sheets (12x12) of absorbent
material, 250 ml and one liter spill control pillows and a small scoop
to collect glass fragments. Absorbents should be suitable for incineration.
Finally, the kit should contain two large CD waste-disposal bags.
(**The person who may use the respirator must be registered in the Respiratory
Protection Program and have current fit test certification.)
- Waste Disposal
Disposal of all CD contaminated materials must be arranged through EHS.
Emergency Response Leaking Compressed Gas Cylinders